6EW4
Human myelin protein P2 F57A mutant, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.760, 36.080, 31.170 |
| Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
| Resolution | 19.112 - 1.271 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.300 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmeas | 0.092 | 0.781 |
| Number of reflections | 31892 | 1880 |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 96.8 | 77.4 |
| Redundancy | 2.9 | 1.8 |
| CC(1/2) | 0.996 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 40-42% PEG 6000 |
