6EW2

Human myelin protein P2 F57A mutant, tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-14
Detector	MAR CCD 165 mm
Wavelength(s)	1.1
Spacegroup name	P 43 21 2
Unit cell lengths	58.612, 58.612, 76.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.302 - 1.588
R-factor	0.1871
Rwork	0.186
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.012
RMSD bond angle	1.332
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2006: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.630
High resolution limit [Å]	1.590	1.590
Rmeas	0.072	1.296
Number of reflections	18475	1258
<I/σ(I)>	19.1	1
Completeness [%]	98.7	92.7
Redundancy	5.7	3.2
CC(1/2)	0.999	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	40-42% PEG 6000