6EW1

Crystal structure of the Filamin A Ig-like domains 3-5 mutant P637Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.961
Spacegroup name	P 65
Unit cell lengths	60.721, 60.721, 163.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.806 - 2.307
R-factor	0.245907158772
Rwork	0.245
R-free	0.27339
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m9p
RMSD bond length	0.003
RMSD bond angle	0.637
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_2313-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.810	2.430
High resolution limit [Å]	2.300	2.310
Rmerge	0.038	0.295
Rmeas	0.040	0.308
Rpim	0.011	0.088
Number of reflections	14953
<I/σ(I)>	36.96	8.3
Completeness [%]	100.0	99
Redundancy	11.9
CC(1/2)	1.000	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05 M ammonium citrate, 18 % PEG 3350