6EVU

Adhesin domain of PrgB from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.975637
Spacegroup name	P 43 21 2
Unit cell lengths	60.550, 60.550, 153.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.516 - 1.598
R-factor	0.1919
Rwork	0.190
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.520	1.700
High resolution limit [Å]	1.590	1.600
Rmeas		1.627
Number of reflections	38693
<I/σ(I)>	28.98
Completeness [%]	99.9	99.6
Redundancy	18.83	16.2
CC(1/2)		0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Bicine/Tris pH 8.5 0.12 M Ethylene Glycol 20% (v/v) Glycerol 10% (w/v) PEG 4000