6EVU
Adhesin domain of PrgB from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975637 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.550, 60.550, 153.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.516 - 1.598 |
| R-factor | 0.1919 |
| Rwork | 0.190 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.520 | 1.700 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Rmeas | 1.627 | |
| Number of reflections | 38693 | |
| <I/σ(I)> | 28.98 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 18.83 | 16.2 |
| CC(1/2) | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Bicine/Tris pH 8.5 0.12 M Ethylene Glycol 20% (v/v) Glycerol 10% (w/v) PEG 4000 |
