6EVS
Characterization of 2-deoxyribosyltransferase from psychrotolerant bacterium Bacillus psychrosaccharolyticus: a suitable biocatalyst for the industrial synthesis of antiviral and antitumoral nucleosides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.547, 107.547, 61.244 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.070 - 1.900 |
| R-factor | 0.1793 |
| Rwork | 0.177 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.927 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.070 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.534 | |
| Rmeas | 0.050 | 0.598 |
| Rpim | 0.020 | 0.267 |
| Number of reflections | 20796 | 2998 |
| <I/σ(I)> | 14.8 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 4.9 |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | in 3 M sodium nitrate, 0.1 M sodium acetate trihydrate, pH 4.6 |
