6EVL
Crystal structure of an unlignaded peptide-substrate-binding domain of human type II collagen prolyl 4-hydroxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-28 |
Detector | Nonius Kappa CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 55.449, 55.449, 71.705 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.000 - 1.870 |
R-factor | 0.1861 |
Rwork | 0.182 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 2V5F |
RMSD bond length | 0.006 |
RMSD bond angle | 0.759 |
Data reduction software | XPREP (Proteum 2 package) |
Data scaling software | SADABS (Proteum 2 package) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.900 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.139 | 0.629 |
Rpim | 0.038 | 0.298 |
Number of reflections | 10985 | 523 |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 14.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2.45 M ammonium sulphate, 10% DMSO, 100 mM MOPS, pH 6.5 |