6EVL

Crystal structure of an unlignaded peptide-substrate-binding domain of human type II collagen prolyl 4-hydroxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-28
Detector	Nonius Kappa CCD
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	55.449, 55.449, 71.705
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.000 - 1.870
R-factor	0.1861
Rwork	0.182
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 2V5F
RMSD bond length	0.006
RMSD bond angle	0.759
Data reduction software	XPREP (Proteum 2 package)
Data scaling software	SADABS (Proteum 2 package)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.900
High resolution limit [Å]	1.870	1.870
Rmerge	0.139	0.629
Rpim	0.038	0.298
Number of reflections	10985	523
<I/σ(I)>	15	2
Completeness [%]	99.9	99.8
Redundancy	14.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2.45 M ammonium sulphate, 10% DMSO, 100 mM MOPS, pH 6.5