6EVG

Structural and Functional Characterisation of a Bacterial Laccase-like Multi-copper Oxidase CueO from Lignin-Degrading Bacterium Ochrobactrum sp. with Oxidase Activity towards Lignin Model Compounds and Lignosulfonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.8
Spacegroup name	P 1 21 1
Unit cell lengths	47.330, 48.310, 88.850
Unit cell angles	90.00, 93.78, 90.00

Refinement procedure

Resolution	88.660 - 1.100
R-factor	0.12636
Rwork	0.125
R-free	0.14926
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pav
RMSD bond length	0.019
RMSD bond angle	1.862
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BALBES
Refinement software	PHENIX (5.8.0171)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.000	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.056	0.584
Number of reflections	161992	11395
<I/σ(I)>	12.2	2.1
Completeness [%]	99.9	99.7
Redundancy	3.72
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.1 M MIB buffer pH 8, 25% PEG 1500