6EVD
Structure of R173A A. niger Fdc1 with prFMN in the hydroxylated form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.230, 64.110, 87.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.850 - 1.190 |
R-factor | 0.14504 |
Rwork | 0.144 |
R-free | 0.16192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4za4 |
RMSD bond length | 0.029 |
RMSD bond angle | 2.564 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.580 | 1.233 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmeas | 0.750 | |
Number of reflections | 168295 | |
<I/σ(I)> | 13.92 | |
Completeness [%] | 97.0 | |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |