6EV9
Structure of E277D A. niger Fdc1 with prFMN in the hydroxylated form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.910, 63.460, 87.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.730 - 1.640 |
R-factor | 0.16767 |
Rwork | 0.166 |
R-free | 0.20494 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4za4 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.896 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.730 | 1.699 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmeas | 1.295 | |
Number of reflections | 65903 | 6502 |
<I/σ(I)> | 7.21 | |
Completeness [%] | 99.0 | |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |