6EV9
Structure of E277D A. niger Fdc1 with prFMN in the hydroxylated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.910, 63.460, 87.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.730 - 1.640 |
| R-factor | 0.16767 |
| Rwork | 0.166 |
| R-free | 0.20494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4za4 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.896 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.730 | 1.699 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmeas | 1.295 | |
| Number of reflections | 65903 | 6502 |
| <I/σ(I)> | 7.21 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |
