6EV8
Structure of E277Q A. niger Fdc1 with prFMN in the hydroxylated form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.910, 63.810, 87.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.080 - 1.030 |
R-factor | 0.14717 |
Rwork | 0.146 |
R-free | 0.16505 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4za4 |
RMSD bond length | 0.032 |
RMSD bond angle | 2.595 |
Data reduction software | iMOSFLM |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.080 | 1.067 |
High resolution limit [Å] | 1.030 | 1.030 |
Rmeas | 0.979 | |
Number of reflections | 246485 | 14510 |
<I/σ(I)> | 12.22 | |
Completeness [%] | 93.0 | |
Redundancy | 5.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |