6EV7
Structure of E282D A. niger Fdc1 with prFMN in the iminium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.760, 63.700, 87.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.080 - 1.060 |
| R-factor | 0.15317 |
| Rwork | 0.152 |
| R-free | 0.16819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4za4 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.465 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 1.098 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmeas | 1.002 | |
| Number of reflections | 240808 | 23555 |
| <I/σ(I)> | 13.99 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |
