6EV6
Structure of E282Q A. niger Fdc1 with prFMN in the hydroxylated and ketimine forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .976 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.050, 63.680, 87.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.070 - 1.100 |
| R-factor | 0.14896 |
| Rwork | 0.148 |
| R-free | 0.16852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4za4 |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.596 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.070 | 1.139 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmeas | 1.002 | |
| Number of reflections | 216927 | 20932 |
| <I/σ(I)> | 11.76 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |
