6EV4
Structure of wild type A. niger Fdc1 purified in the dark with prFMN in the iminium form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.970, 64.030, 87.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.980 - 1.140 |
| R-factor | 0.14965 |
| Rwork | 0.149 |
| R-free | 0.16732 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.417 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 1.181 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmeas | 0.807 | |
| Number of reflections | 186270 | 18286 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 94.9 | |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |
