6EV3
Structure of wild type A. niger Fdc1 that has been illuminated with UV light, with prFMN in the iminium and ketimine form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.131, 63.906, 87.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.850 - 1.300 |
R-factor | 0.17424 |
Rwork | 0.173 |
R-free | 0.19711 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4za4 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.244 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.850 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmeas | 1.223 | |
Number of reflections | 475106 | 12466 |
<I/σ(I)> | 7.4 | 1.1 |
Completeness [%] | 96.4 | |
Redundancy | 3.7 | 3.7 |
CC(1/2) | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |