6EV1
Crystal structure of antibody against schizophyllan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.084, 112.832, 140.816 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 56.416 - 3.043 |
| R-factor | 0.1882 |
| Rwork | 0.186 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mim |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.12rc1_2807: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.416 | 3.096 |
| High resolution limit [Å] | 3.040 | 3.043 |
| Rmerge | 0.130 | 0.647 |
| Rmeas | 0.155 | 0.764 |
| Rpim | 0.083 | 0.404 |
| Number of reflections | 55718 | 2768 |
| <I/σ(I)> | 8.9 | 2.2 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris, lithium sulfate, PEG 4000 |
