6EUQ
MdfA(Q131R/L339E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.189, 62.869, 100.480 |
| Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
| Resolution | 44.640 - 2.200 |
| R-factor | 0.23166 |
| Rwork | 0.231 |
| R-free | 0.24833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zow |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.640 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.135 | 0.661 |
| Number of reflections | 28294 | 2449 |
| <I/σ(I)> | 8 | 0.9 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291.15 | 50 mM sodium acetate, pH 5.6, 20%-28% (v/v) PEG 400, 10 mM praseodymium acetate, and 50 mM magnesium acetate |
