6EUN
Crystal structure of Neisseria meningitidis vaccine antigen NadA variant 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.761, 39.778, 255.494 |
| Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
| Resolution | 34.840 - 2.450 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.840 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.378 |
| Rmeas | 0.062 | 0.474 |
| Number of reflections | 23543 | 3487 |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 91.3 | 94.2 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium phosphate citrate pH 3.9, 5% (w/v) PEG 1K, 33.6-45.9% (v/v) MPD |
