6EUJ
The GH43, Beta 1,3 Galactosidase, BT0265
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.321, 73.331, 116.457 |
| Unit cell angles | 91.04, 107.41, 99.93 |
Refinement procedure
| Resolution | 110.810 - 2.750 |
| R-factor | 0.21791 |
| Rwork | 0.216 |
| R-free | 0.25414 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 110.810 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 58162 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 98.4 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % PEG 8K 0.1M Hepes pH 7.5 |
