6EUD
Crystal structure of E. coli DExH-box NTPase HrpB
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763, 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.710, 131.410, 166.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.623 - 2.400 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.23860 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.087 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.200 | 1.560 |
Number of reflections | 43551 | 3189 |
<I/σ(I)> | 11.7 | 1.79 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.1 | 13.6 |
CC(1/2) | 0.997 | 0.584 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES-NaOH, pH 6.5, 5% [w/v] PEG 6000, 10 % [v/v] MPD, 0.1 M LiCl |