6EU7
Structure of the arsenite-bound form of AioX from Rhizobium sp. str. NT-26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.917 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.359, 116.917, 136.838 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 136.840 - 3.000 |
| R-factor | 0.19344 |
| Rwork | 0.192 |
| R-free | 0.21495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6esk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.659 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.838 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.587 |
| Number of reflections | 62521 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 97.9 | |
| Redundancy | 4 | |
| CC(1/2) | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 289.15 | 25 % PEG 3350, 0.1 M Tris-HCl |
