6ETH
The Structure of the Mo-insertase domain Cnx1E from Arabidopsis thaliana in complex with AMP and tungstate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2094 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.500, 123.470, 133.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.580 - 1.640 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g2r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS (Nov 1, 2016) |
Data scaling software | STARANISO (1.0.5) |
Phasing software | PHASER (2.6.0) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.582 | 1.770 |
High resolution limit [Å] | 1.637 | 1.637 |
Rmerge | 0.057 | 1.864 |
Rmeas | 0.061 | 1.980 |
Rpim | 0.022 | 0.665 |
Number of reflections | 50634 | 2532 |
<I/σ(I)> | 21.7 | |
Completeness [%] | 75.7 | 18.4 |
Redundancy | 14.89 | 17.05 |
CC(1/2) | 0.999 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 20 % (v/v) 1,2-Ethanediol; 10 % (w/v) PEG 8000; 0.3 M MgCl2; 0.3 M CaCl2; 0.1 M Tris/BICINE, pH 7.4 |