6ETC
Crystal Structure of Human gamma-D-crystallin Mutant P23T+R36S at 1.2 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.020, 31.700, 52.500 |
| Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
| Resolution | 44.009 - 1.197 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.17450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jgf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.010 | 1.240 |
| High resolution limit [Å] | 1.197 | 1.197 |
| Rmerge | 0.077 | 1.140 |
| Rmeas | 0.101 | 1.501 |
| Number of reflections | 45768 | |
| <I/σ(I)> | 8.93 | 1 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 1.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 310 | 0.1M phosphate buffer, 20mM DTT |
