6ETB
Aerobic S262Y mutation of E. coli FLRD core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 89.383, 64.407, 147.254 |
Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
Resolution | 75.758 - 1.905 |
R-factor | 0.1998 |
Rwork | 0.200 |
R-free | 0.19980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d02 |
RMSD bond length | 0.018 |
RMSD bond angle | 0.560 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1436) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.830 | |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.113 | |
Rmeas | 0.137 | 0.863 |
Rpim | 0.075 | 0.431 |
Number of reflections | 57099 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 80.7 | 88.7 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.15 | 1.0 UL OF PROTEIN AT 15 MG/ML WITH 2.0 UL OF CRYSTALLIZATION SOLUTION (0.1 M SODIUM CITRATE PH 5.6, 20% (W/V) PEG 4000 AND 20% (V/V) 2-PROPANOL) |