6ETA
Crystal Structure of Human Gamma-D crystallin Mutant P23T+R36S at Room Temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.036, 82.103, 106.248 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.165 - 2.198 |
R-factor | 0.2344 |
Rwork | 0.233 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jgf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.700 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.165 | 2.270 |
High resolution limit [Å] | 2.198 | 2.198 |
Rmerge | 0.129 | 1.290 |
Rmeas | 0.139 | 1.430 |
Number of reflections | 24656 | |
<I/σ(I)> | 9.2 | |
Completeness [%] | 99.6 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 293 | 0.1M phosphate, 20mM DTT |