6ESV
Structure of the phosphate-bound form of AioX from Rhizobium sp. str. NT-26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.978 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.544, 124.544, 48.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.070 - 1.780 |
R-factor | 0.19189 |
Rwork | 0.190 |
R-free | 0.22038 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 2.008 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 88.070 |
High resolution limit [Å] | 1.780 |
Number of reflections | 36718 |
<I/σ(I)> | 19.3 |
Completeness [%] | 99.8 |
Redundancy | 16.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289.15 | 0.49 M NaH2PO4 and 0.91 M K2HPO4 |