6ESL
Crystal structure of the Legionella pneumoppila LapA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.597, 99.556, 104.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.560 - 1.870 |
| R-factor | 0.19471 |
| Rwork | 0.192 |
| R-free | 0.23765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gne |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.045 |
| Data reduction software | Aimless |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.550 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 66414 | 4808 |
| <I/σ(I)> | 12.5 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 0.1M MES pH 5.5, 25% (w/v) PEG 4000 |
