6ESK
Structure of the apo form of AioX from Rhizobium sp. str. NT-26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.987 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.877, 124.877, 47.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.300 - 1.750 |
R-factor | 0.18854 |
Rwork | 0.187 |
R-free | 0.21115 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6esv |
RMSD bond length | 0.022 |
RMSD bond angle | 1.969 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.590 |
High resolution limit [Å] | 1.750 |
Number of reflections | 38474 |
<I/σ(I)> | 7.6 |
Completeness [%] | 98.9 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20% PEG 3350, 0.2 M sodium malonate and 0.1 M Bis-Tris propane, pH 8.5 |