6ESG

Nucleosome breathing : Class 2

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K2 SUMMIT (4k x 4k),FEI FALCON II (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	5.4
Software	RELION,PHENIX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION