6ESC

Crystal structure of Pseudorabies virus glycoprotein B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	H 3
Unit cell lengths	99.890, 99.890, 272.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.150 - 2.700
R-factor	0.195
Rwork	0.193
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gum
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150	2.850
High resolution limit [Å]	2.690	2.690
Rmerge	0.074	0.523
Rpim	0.055	0.386
Number of reflections	28086
<I/σ(I)>	14.99	2.33
Completeness [%]	99.6	98.3
Redundancy	5.3
CC(1/2)	0.980	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris pH 8.5, 7% PEG 4,000, 0.6M LiCl