6ES4
A cryptic RNA-binding domain mediates Syncrip recognition and exosomal partitioning of miRNA targets
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.783, 128.528, 79.585 |
| Unit cell angles | 90.00, 101.12, 90.00 |
Refinement procedure
| Resolution | 37.561 - 2.200 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.20550 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.045 | 0.421 |
| Number of reflections | 33579 | 5270 |
| <I/σ(I)> | 19.15 | 3.31 |
| Completeness [%] | 99.0 | 96.8 |
| Redundancy | 3.36 | 3.2 |
| CC(1/2) | 0.999 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 2.2 M ammonium sulphate, 0.1 M sodium citrate pH 6, Seeding |
