6ERY
The crystal structure of mouse chloride intracellular channel protein 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-07-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.623, 68.989, 80.980 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 40.327 - 1.795 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.906 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.327 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 2.955 |
| Number of reflections | 50630 | |
| <I/σ(I)> | 12.82 | 0.42 |
| Completeness [%] | 98.8 | 87.4 |
| Redundancy | 5.23 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | PEG 3350, LiSO4, Bis-Tris |
