6ERQ
Structure of the human mitochondrial transcription initiation complex at the HSP promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 103.620, 197.730, 134.340 |
Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
Resolution | 49.576 - 4.500 |
R-factor | 0.2932 |
Rwork | 0.291 |
R-free | 0.33340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Model of the human mitochondrial IC on the LSP promoter |
RMSD bond length | 0.002 |
RMSD bond angle | 0.513 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.580 | 4.610 |
High resolution limit [Å] | 4.500 | 4.500 |
Rmeas | 0.087 | 2.232 |
Number of reflections | 31460 | 2303 |
<I/σ(I)> | 13.38 | 1.11 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 7 | 7.3 |
CC(1/2) | 1.000 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | L-Proline, PEG8000, BIS-TRIS |