6ER8
Enterococcus faecalis FIC protein in complex with phosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.130, 65.130, 248.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.910 - 2.292 |
R-factor | 0.2326 |
Rwork | 0.229 |
R-free | 0.29870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g03 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.893 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.910 | 2.380 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.312 | 1.808 |
Number of reflections | 23660 | 2338 |
<I/σ(I)> | 5.2 | |
Completeness [%] | 94.8 | 91.7 |
Redundancy | 4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50mM Tris pH 8.0, 200mM NaCl, 1mM MgCl2, 0.2M Ammonium sulfate, 0.1M Tris-sodium citrate pH 5.6, 15% (w:v) PEG 4000 |