6ER7
CHEMOTAXIS PROTEIN CHEY FROM Pyrococcus horikoshiI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9334 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 53.160, 65.730, 72.890 |
Unit cell angles | 90.00, 110.94, 90.00 |
Refinement procedure
Resolution | 34.038 - 2.620 |
Rwork | 0.212 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tmy |
RMSD bond length | 0.012 |
RMSD bond angle | 1.652 |
Data reduction software | xia2 (0.3.8) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0158 2016/10/03) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.030 |
Rmeas | 0.030 |
Rpim | 0.020 |
Number of reflections | 107515 |
<I/σ(I)> | 38 |
Completeness [%] | 99.5 |
Redundancy | 7.6 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris-HCl 1.2 M sodium malonate |