6ER6
Crystal structure of a computationally designed colicin endonuclease and immunity pair colEdes7/Imdes7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-05-24 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.340, 56.297, 101.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.510 - 1.600 |
| R-factor | 0.1994 |
| Rwork | 0.197 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u43 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.990 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.051 | 0.460 |
| Number of reflections | 32715 | 3130 |
| <I/σ(I)> | 26.8 | 6 |
| Completeness [%] | 99.6 | 97.84 |
| Redundancy | 3.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 12% PEG 1,500 and 0.05M MMT buffer pH=8.0 |
