6EQT

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE PROTEIN CENP-N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97793
Spacegroup name	P 41
Unit cell lengths	87.300, 87.300, 81.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.300 - 2.735
R-factor	0.208
Rwork	0.206
R-free	0.25380
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	XSCALE
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.300	87.300	2.810
High resolution limit [Å]	2.735	12.230	2.735
Rmerge	0.077	0.044	1.461
Rmeas	0.082	0.047	1.551
Total number of observations	152272
Number of reflections	16182	191	1172
<I/σ(I)>	17.28	46.41	1.37
Completeness [%]	99.8	97.9	98.5
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	6-16% PEG3350, pH 6.6-7.2