6EPI
Structure of the epsilon_1 / zeta_1 antitoxin / toxin system from Neisseria gonorrhoeae in complex with UNAM-4P.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.510, 149.320, 125.440 |
| Unit cell angles | 90.00, 94.36, 90.00 |
Refinement procedure
| Resolution | 49.170 - 2.800 |
| R-factor | 0.22043 |
| Rwork | 0.219 |
| R-free | 0.24359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6epg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.113 | 0.920 |
| Number of reflections | 68024 | |
| <I/σ(I)> | 11.3 | 1.9 |
| Completeness [%] | 99.7 | 89.6 |
| Redundancy | 4.1 | 3.8 |
| CC(1/2) | 0.997 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 100 mM sodium citrate pH 4.5, 1.8 M (NH4)2SO4 |
