6EP5

Enterococcus faecalis FIC protein in complex with ADP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976251
Spacegroup name	P 43 21 2
Unit cell lengths	87.836, 87.836, 364.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.301 - 1.928
R-factor	0.2024
Rwork	0.200
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5NUW
RMSD bond length	0.007
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.172
Rpim	0.071
Number of reflections	108913	5168
<I/σ(I)>	10.35
Completeness [%]	99.9	99.2
Redundancy	13	12.6
CC(1/2)	0.998	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50mM Tris pH 8.0, 100mM NaCl, 5mM ATPgS, 2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5