6EOG

Human galectin-3c in complex with a galactose derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-19
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	36.080, 58.440, 62.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.730 - 1.200
R-factor	0.11376
Rwork	0.112
R-free	0.14624
RMSD bond length	0.023
RMSD bond angle	2.384
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.200
Rmerge	0.033
Number of reflections	42181
<I/σ(I)>	29.7
Completeness [%]	99.6
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	19 MG/ML GALECTIN-3C, 100MM NACL, 29 % W/V PEG 4000, 0.4 M NASCN, 18 MM BETA-MERCAPTOETHANOL,0.02 % NAN3, 10 MM PHOSHATE, 100 MM MGCL2, 100 MM TRIS/HCL PH 7.5, 2.5 MM LIGAND