6ENT
Structure of the rat RKIP variant delta143-146
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 111.250, 111.250, 111.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.660 |
| R-factor | 0.21148 |
| Rwork | 0.210 |
| R-free | 0.25043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ens |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.322 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.630 | 2.810 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.106 | 1.465 |
| Number of reflections | 6706 | |
| <I/σ(I)> | 14.7 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 3350, sodium fluoride |
