6ENT

Structure of the rat RKIP variant delta143-146

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-23
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	I 2 3
Unit cell lengths	111.250, 111.250, 111.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.660
R-factor	0.21148
Rwork	0.210
R-free	0.25043
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ens
RMSD bond length	0.009
RMSD bond angle	1.322
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.630	2.810
High resolution limit [Å]	2.660	2.660
Rmerge	0.106	1.465
Number of reflections	6706
<I/σ(I)>	14.7	1.8
Completeness [%]	100.0	100
Redundancy	12.1	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG 3350, sodium fluoride