6ENR
The ENTH domain from epsin Ent2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2395 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.395, 65.726, 33.735 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 33.460 - 1.840 |
| R-factor | 0.16795 |
| Rwork | 0.165 |
| R-free | 0.22152 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.042 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.000 |
| High resolution limit [Å] | 1.840 |
| Number of reflections | 8733 |
| <I/σ(I)> | 23.6 |
| Completeness [%] | 99.2 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8 mg/ml of ENTH2 in a buffer containing 10 % (v/v) isopropanol, 20 % PEG 4000 and 0.1 M Hepes pH 7.5 |
