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6EN0

Structure of the Tn1549 transposon Integrase (aa 82-397) in complex with circular intermediate DNA (CI5-DNA)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP13 (MX1)
Temperature [K]100
Detector technologyPIXEL
Collection date2015-09-21
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.9763
Spacegroup nameP 1 21 1
Unit cell lengths77.660, 124.320, 77.400
Unit cell angles90.00, 117.69, 90.00
Refinement procedure
Resolution46.044 - 2.800
R-factor0.2283
Rwork0.227
R-free0.25430
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6emz
RMSD bond length0.003
RMSD bond angle0.579
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]46.0442.870
High resolution limit [Å]2.8002.800
Rmeas0.1251.739
Number of reflections311282363
<I/σ(I)>10.670.95
Completeness [%]96.698.5
Redundancy2.852.87
CC(1/2)0.9950.622
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1M Na Acetate pH4.6, 30% PEG300

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