6EMZ
Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with circular intermediate DNA (CI5-DNA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.590, 125.060, 77.980 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 48.357 - 2.790 |
R-factor | 0.1994 |
Rwork | 0.197 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6emy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.399 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.360 | 2.670 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 40207 | |
<I/σ(I)> | 13.29 | 1.27 |
Completeness [%] | 99.8 | 79.3 |
Redundancy | 5.6 | 7.3 |
CC(1/2) | 0.998 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M NH4-fluoride, 14% PEG3350 |