6EMZ

Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with circular intermediate DNA (CI5-DNA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	64.590, 125.060, 77.980
Unit cell angles	90.00, 96.53, 90.00

Refinement procedure

Resolution	48.357 - 2.790
R-factor	0.1994
Rwork	0.197
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6emy
RMSD bond length	0.002
RMSD bond angle	0.399
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	2.670
High resolution limit [Å]	2.520	2.520
Number of reflections	40207
<I/σ(I)>	13.29	1.27
Completeness [%]	99.8	79.3
Redundancy	5.6	7.3
CC(1/2)	0.998	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M NH4-fluoride, 14% PEG3350