6EMY

Structure of the Tn1549 transposon Integrase (aa 82-397, Y379F) in complex with transposon right end DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977315
Spacegroup name	P 1 21 1
Unit cell lengths	77.230, 121.681, 77.234
Unit cell angles	90.00, 118.12, 90.00

Refinement procedure

Resolution	38.597 - 2.500
R-factor	0.1625
Rwork	0.161
R-free	0.18390
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.380	2.870	2.640
High resolution limit [Å]	2.500	2.800	2.500
Rmerge	0.065		0.835
Rpim	0.039		0.493
Number of reflections	41916	4618	6187
<I/σ(I)>	11.7	1.35	1.6
Completeness [%]	96.5	98.2	98
Redundancy	3.8	3.5	3.8
CC(1/2)	0.999	0.547	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	13% (v/v) PEG 3350, 0.25 M NaCl
2	VAPOR DIFFUSION		293	13% (v/v) PEG 3350, 0.25 M NaCl