6EMU
Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 61 |
| Unit cell lengths | 71.084, 71.084, 191.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.363 - 2.297 |
| R-factor | 0.196891010953 |
| Rwork | 0.195 |
| R-free | 0.22273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Crystal structure of TkTrm10 construct ( to be deposited) |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.525 |
| Data reduction software | XDS (2015-10-15) |
| Data scaling software | XDS (2015-10-15) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.363 | 2.400 |
| High resolution limit [Å] | 2.297 | 2.300 |
| Rmerge | 0.113 | 1.946 |
| Rmeas | 0.116 | 2.000 |
| Rpim | 0.025 | 0.437 |
| Number of reflections | 24345 | 2406 |
| <I/σ(I)> | 20.55 | 1.45 |
| Completeness [%] | 99.8 | 98.12 |
| Redundancy | 21 | 20.4 |
| CC(1/2) | 1.000 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 10% PEG6000, 0.2M LiCl and 0.1M NaAc pH 5.5 |
