6ELM

Crystal structure of the human WNK2 CCT1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.928
Spacegroup name	P 21 21 21
Unit cell lengths	28.197, 46.492, 71.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.944 - 1.140
R-factor	0.1397
Rwork	0.138
R-free	0.16830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o2c
RMSD bond length	0.015
RMSD bond angle	1.297
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.490
High resolution limit [Å]	1.140
Number of reflections	35275
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	30% PEG1000 -- 0.1M SPG pH 8.0