6ELE
FAB Fragment. AbVance: Increasing our knowledge of antibody structural space to enable faster and better decision making in antibody drug discovery
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 51.417, 74.131, 76.515 |
Unit cell angles | 101.93, 102.23, 111.27 |
Refinement procedure
Resolution | 17.940 - 1.780 |
R-factor | 0.191 |
Rwork | 0.190 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in house fAB |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.320 |
High resolution limit [Å] | 1.780 |
Number of reflections | 90969 |
<I/σ(I)> | 8.24 |
Completeness [%] | 97.0 |
Redundancy | 3.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M TRIS, pH 9.0, 22.5 %w/v PEG 6K |