6EKL
Crystal structure of mammalian Rev7 in complex with human Chromosome alignment-maintaining phosphoprotein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-19 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.280, 51.840, 126.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.410 - 1.600 |
Rwork | 0.233 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o8k |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 41402 | 5971 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.5 M ammonium sulfate, 0.1 M Tris-Bicine pH 8.5, 0.15 M lithium sulfate, 0.02 M sodium nitrate, 0.02 M sodium phosphate dibasic, 1.5% v/v MPD, 1.5% PEG 1000, 1.5% w/v PEG 3350 |