6EKH

Crystal structure of activated CheY from Methanoccocus maripaludis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 31 2 1
Unit cell lengths	71.332, 71.332, 77.115
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.670 - 2.654
R-factor	0.2859
Rwork	0.284
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ekg
RMSD bond length	0.005
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.210	2.810
High resolution limit [Å]	2.650	2.650
Rmerge	0.037	0.183
Number of reflections	6584	659
<I/σ(I)>	14.3	2.8
Completeness [%]	92.1	94.7
Redundancy	1.9	1.9
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M NaCl, 0.1 M HEPES pH 7.5, 20 % PEG 3000