6EKG

Crystal structure of an archaeal CheY from Methanoccocus maripaludis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 41 21 2
Unit cell lengths	44.096, 44.096, 123.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.544 - 1.150
R-factor	0.1545
Rwork	0.154
R-free	0.17220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u0s
RMSD bond length	0.012
RMSD bond angle	1.198
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.100	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.047	0.755
Number of reflections	44463	4361
<I/σ(I)>	14	1.9
Completeness [%]	99.9	99.9
Redundancy	6.3	5.6
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Magnesium formate 20 % PEG 3350